FastQCFastQC Report
Qua 1 nov 2017
BcaribensisP30_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBcaribensisP30_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931205
Sequences flagged as poor quality0
Sequence length35-251
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19520.20962086758554774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACA1151.2993041E-859.7405241
TATAACT1000.00137670439.2580574
ACCTTAA1401.695419E-435.0518341
ATAGTTA1150.002734100434.1374443
GTTCTAG1452.083655E-433.8431551
TAGTACT1400.007157773228.041474
TATATGT1450.00849455827.0745244
GTATACC1450.00849455827.0745244
TAGAGCT2000.001367760524.5362854
GTCCTAT2451.5773195E-424.0355451
CACAATA2300.003082245821.3359012
CACTCAT3755.549675E-620.937631
TAGACCG3006.200146E-419.629035
GTATAGG3056.9298706E-419.3072411
GTAGACA2600.00626638318.8740654
CCCACGG13601.39249205E-518.813911245
TACTTGT2650.006994640518.5179527
GTGTACT3901.7734538E-417.6157951
CTTTATG3901.7734538E-417.6157951
GTCTACA4354.079523E-415.7934711
Produced by FastQC (version 0.11.4)