FastQCFastQC Report
Qua 1 nov 2017
BcaribensisP30_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBcaribensisP30_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931205
Sequences flagged as poor quality0
Sequence length35-251
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19440.20876176566921356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT953.957939E-8143.41196245
ACTTATA1600.007737132351.090508245
TAAACAA3400.003717412532.05679245
ATATATA1902.523613E-531.4586541
GCCTAGA1400.006655344728.4625912
TAAAAGT1500.00931895826.5650844
GGCCTAA1500.00931895826.5650841
CTTATGA2050.001447998724.2973351
TGTAAGA3351.8321552E-623.7896292
GTGTAAG2951.8450295E-523.6384221
GGTGGGG19351.8189894E-1221.122692245
GGGGAGG14258.762072E-921.033754245
AATAAAA5604.0896877E-519.46305245
TACACAG4151.1714614E-519.2036765
TATTGGG4201.2989296E-518.9750612
TTAGCTC3208.657754E-418.6785764
GGTATAG3208.657754E-418.6785761
GAGGAGG8805.21919E-718.578367245
CCCCCAG7350.006946062718.536238245
CCCGGGG14701.8644641E-918.536238245
Produced by FastQC (version 0.11.4)