FastQCFastQC Report
dom 14 out 2018
bcp30_r2_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamebcp30_r2_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922122
Sequences flagged as poor quality0
Sequence length1-251
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTAA1004.9691254E-512180.546245
ATTTTGT1851.70062E-46584.078245
CTAGTTA152.4871188E-45718.5654230-234
TTCCCTT3506.0865126E-43480.1558245
TTTGTTT4459.838624E-42737.2014245
TGGACAA5400.0014487182255.6565245
CTAAAAT700.0023643421851.1467240-244
TCAACAG6700.00223008381817.9917245
GTACGTG6950.00239958661752.5964245
CCTAAAA1400.00265044581740.0779240-244
TTGGAGT1750.0041409411392.0623240-244
CACCATG9200.00420435841323.9723245
TTAATGC2000.00540822051218.0544240-244
GAAACTA1200.0056738841194.171235-239
GTAGCTA300.00645435281124.7041220-224
ATACAAC2300.0071518081059.1779240-244
TAAAATA850.0073197511053.6803240-244
CACTTAA500.00745702951045.5403235-239
CTCTTAT1450.008283324988.2796235-239
CAGTAAG1350.008791028959.8538240-244
Produced by FastQC (version 0.11.5)