FastQCFastQC Report
Qua 1 nov 2017
CupriavidusP15_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCupriavidusP15_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545430
Sequences flagged as poor quality0
Sequence length35-251
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29060.1880382806079861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGA906.4782234E-445.758171
TCTAGAA1351.0333023E-438.131812
GTATAAT1100.001738576237.4385031
GTCTAGA1401.2799414E-436.769961
CTAAGGG3450.002027104436.281162245
TACTCAC2050.001195415725.111192
TATACAG2951.4304291E-524.4302125
TCTATAG2150.001577701823.943232
GTGCTAT4902.9740477E-923.1125451
GTACATA2300.002335393322.3817141
GTCTTTT7550.003646862520.72351245
CCTACAC8300.019.8469183
CTTGAAA13051.1805934E-519.183144245
GGTTATA2700.005912613619.0659051
TATGACT5452.308825E-718.8909874
TATAGAA2750.00657346618.7192522
ATATACA3901.2300705E-418.4792614
GTATAGG6154.0632585E-818.4148751
TATTACG2800.007293700718.384982
ATAGGAC4501.7731989E-518.3032683
Produced by FastQC (version 0.11.4)