FastQCFastQC Report
dom 14 out 2018
cup15_r1_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecup15_r1_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503233
Sequences flagged as poor quality0
Sequence length1-251
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAGG2209.927195E-6272.26828245
AAAATAT2550.0056209993156.59875245
TATTGGG5402.9949668E-536.974705245
TAGACAG2256.526534E-933.7016145
CTGACGG25950.00451037430.776632245
ATATTAT1653.066004E-530.5765861
TTCTATA2404.3728687E-728.0388152
CCCTAAA1506.292188E-428.0285361
TCTATAC3404.4383341E-1027.2277573
TTATAGA2051.3408014E-424.6194462
TATACCG5850.024.484085
TTATGCT1750.001535507624.0549144
CTACACT2151.8407972E-423.4954994
TTATACC1800.001809076523.3867234
TATAAGG2202.1641908E-422.940852
TAGGGTC1900.002477082722.1558424
GTACACG9300.004539457621.469183245
TATAGAT2403.8977788E-421.0291122
GTCTATA3205.3647127E-621.0214021
GTATACC2050.00384892420.5346854
Produced by FastQC (version 0.11.5)