FastQCFastQC Report
Qua 1 nov 2017
CupriavidusP15_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCupriavidusP15_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545430
Sequences flagged as poor quality0
Sequence length35-251
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28440.18402645218482883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTA1500.00974714548.19554245
TAAACAA5352.1827873E-1145.04256245
CACTTAA2208.019672E-443.81413245
TCTAACA2600.001823555937.073494245
CGCTTAA4104.7669548E-429.387526245
TAACACA4401.3279809E-527.383831245
AAAATAA4457.6968124E-427.076147245
GTCTTAA2551.1862145E-425.0677471
TACACAA7801.313183E-624.715662245
TGTGAGG6703.270476E-421.580095245
ATAGAAA6803.6147627E-421.26274245
AGACAGA8155.1430034E-520.697472245
GTATAGA5901.7051207E-819.8630031
ACAAATA7406.393257E-419.538733245
AAAAAGA12551.9410436E-719.201412245
TATAGGA2850.006631299418.6908654
GGTTATA2900.00733127718.3686081
ATAGGAT2950.008090693518.0572745
GAGAGAA9752.0346245E-417.300964245
AACACAA9852.1996001E-417.125319245
Produced by FastQC (version 0.11.4)