FastQCFastQC Report
dom 14 out 2018
cup15_r2_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamecup15_r2_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503233
Sequences flagged as poor quality0
Sequence length1-251
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA1601.8447481E-647120.656235-236
GCACTCT2008.647185E-625131.016235-236
CGCACTC7003.530965E-510770.436235-236
TATCCCT2906.060056E-510399.042230-234
TCCCTGT3305.178521E-43807.7302230-234
ACAGATA2005.4757274E-43769.6526225-229
AGCTAAT1406.5664167E-43474.334220-224
TGTCTCT2906.362243E-43466.347230-234
ATTAGGC1507.537881E-43242.7117220-224
GAATTAG1009.1857254E-42956.5903215-219
ATCCCTG5808.72525E-42888.6226230-234
TAAATCG1709.6817414E-42861.216220-224
GTCTCTT3159.722247E-42815.8230-234
TTTACAG650.00111251672697.426220-224
GTTATTA400.00112156422692.6091215-219
CTAATCT700.00129023232504.7524220-224
CTACAGA1800.00130946712463.8252225-229
CTGTCTC5250.00131056662393.4302230-234
ATTTTAT1300.00155228642274.3003215-219
GCTGTCT7500.00145888672233.8682230-234
Produced by FastQC (version 0.11.5)