FastQCFastQC Report
Qua 1 nov 2017
HerbaspirillumP19_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHerbaspirillumP19_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407873
Sequences flagged as poor quality0
Sequence length35-251
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGG859.108723E-487.63174245
GACTGTT1300.004912178557.297672245
TAAGACA853.400101E-452.1384664
ATAAGAC955.8851414E-446.6502083
GTATAAG1250.002269439435.4541551
GTTATAG1400.003954814331.6554953
AGTAGAC1450.004695680530.5639273
AGAATAT1500.00554227229.5451283
GAATAAG1550.006505238428.5920581
TGTACGA2057.780299E-427.0229852
AGACATT2250.001339297424.6209416
TGGTACC3452.7160735E-522.4799924
TACTGGA3002.7320365E-422.1588462
ACTAAGG4501.5439582E-822.070215245
GTCTTAT1900.004882164819.705807240-244
TGCAAGA4651.2462684E-519.0613752
GTACTGC5251.6678787E-618.9932986
ATCCAAG3000.0071029218.4657061
TGGTACT3609.3385624E-418.4657064
GCTGTGG8550.001374791117.423853245
Produced by FastQC (version 0.11.4)