FastQCFastQC Report
dom 14 out 2018
hbp19_r1_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehbp19_r1_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397794
Sequences flagged as poor quality0
Sequence length1-251
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCAT500.00143177952363.6814245
GTTTGTA250.00391168941441.678240-244
TATTTGT850.0041370641390.4009245
CATAAGA300.00563231861201.3983240-244
GACTCGA1050.00631227951125.5626245
GTTGATA1150.0075714661027.6875245
CGCGTAA1200.0082439445984.86725245
ACGTGAG654.126706E-4374.0828220-224
ATTGACT900.002033843219.71098230-234
GGGTAAT450.0032990882187.1275220-224
CTCAGAA900.005491862157.72548235-239
ATGATAA550.0065497304148.78978190-194
CTTTCTG1852.1850118E-4125.2423225-229
ACCATGC5950.00825644160.574707240-244
CCTCCTC2750.005706983655.10842215-219
GGTGTGC3850.003377549439.520298225-229
GTACAAG1700.006047549739.179054205-209
ACTGTGC1650.004625303739.01138220-224
TAGTACA750.002587628634.5180172
TGCTACA800.00355025332.360642
Produced by FastQC (version 0.11.5)