FastQCFastQC Report
Qua 1 nov 2017
HerbaspirillumP19_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHerbaspirillumP19_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407873
Sequences flagged as poor quality0
Sequence length35-251
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTG1100.003690517761.591072245
GTAATAT550.00377134861.2663151
TGAGAGG1854.6935125E-448.82896245
GATTAGA700.00979059348.137821
CAACCAA2604.784487E-543.429607245
ACACAGA2500.002065186936.133434245
TATTGAC2152.5829599E-531.3455582
GTGGGTT3050.00546992229.617569245
TAGAGCT2304.0934246E-529.3012834
CTATATC1550.00608441228.9862164
TACAGTG1550.00608441228.9862165
TCCGAGT1650.00825602127.2294753
TAATCCG1650.00825602127.2294755
GTATGCA2300.001405549924.4177361
AAAACAA5200.002775580921.714804245
GATCTAT2600.00286697121.6003041
GGTGGGA5300.003099916721.305088245
ATAGAGC2800.00440490720.0574253
CAGTTTA700.004745209619.805454235-239
CAGGGGG10751.7333568E-618.907028245
Produced by FastQC (version 0.11.4)