FastQCFastQC Report
dom 14 out 2018
hbp19_r2_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehbp19_r2_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397794
Sequences flagged as poor quality0
Sequence length1-250
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGAGC1758.47018E-58029.657225-228
TTTCTAG401.3422572E-47527.8037220-224
GGTCTTA451.4561058E-47206.1025215-219
CATGTTT1052.1343674E-45735.4697220-224
TAAATCA1102.342471E-45474.766220-224
TTTTCTA1102.342471E-45474.766220-224
TTCTAGC402.8024084E-45269.4624220-224
AAATAAA1202.7877121E-45018.5356220-224
GTCTTAA404.4247156E-44215.5703215-219
TAAATGA455.599929E-43747.173220-224
ATGTCCG2355.090901E-43587.7192225-228
TATAAAA1007.1899587E-43242.746215-219
GTCCGCA1957.360866E-43088.3298225-228
TCTAGCA558.365022E-43065.8691220-224
TGTGTTT458.6795195E-43021.9138205-209
TGTCCGC2407.9644384E-42927.4792225-228
TCTTAAA409.822123E-42848.3582215-219
CATGTCC5157.335128E-42728.5242225-228
ATAAGCA500.00107152112719.7227205-209
AATAAAT1150.00110930162618.3665220-224
Produced by FastQC (version 0.11.5)